![]() On day 6 post treatment, the tumors in rats treated with all doxorubicin formulations exhibited an increase of cell death rate, decreased proliferative activity, and a lower indicator of CRC of tumor cells, compared to untreated animals. The number and volume fraction of blood vessels in tumors were determined on histological sections stained with isolectin B4. The number of mitotically dividing and dying tumor cells was counted, and the tumor cell renewal coefficient (CRC) was calculated for evaluation of pathomorphosis of glioblastoma 101.8 induced by doxorubicin, on day 14 post transplantation. Lyophilized nanoparticles were resuspended in water (DOX-PLGA) or in 1% aqueous solution of poloxamer 188 (DOX-PLGA/P188) before intravenous administration. The study was performed on 33 male Wistar rats receiving doxorubicin (DOX) substance or doxorubicin-loaded nanoparticles (DOX-PLGA) at a dose of 1.5 mg/kg (body weight) on days 2, 5, and 8 post tumor transplantation. Doxorubicin bound to poly(lactic-co-glycolic acid) (PLGA) nanoparticles was used in the investigation. The aim of the investigation was to study drug-induced pathomorphosis of glioblastoma 101.8 in Wistar rats treated with different doxorubicin formulations: doxorubicin bound to polylactide nanoparticles and doxorubicin substance. © 2017, Nizhny Novgorod State Medical Academy. The application of LILT using NeHe laser-based MACDEL-08 device is a harmless, clinically effective, physiologically justified and technically simple component of primary open-angle glaucoma complex treatment. The analysis of rheographic parameters has shown the reduction in the deficit of eye tissue blood supply and improvement of elastic properties of the intraocular vessel walls during LILT and after it. Upon completion of the LILT course, improvement of visual acuity has been noted in all patients, in group 1 from 0.4–0.9 to 0.5–1.0 in group 2 from 0.1–0.8 to 0.3–1.0 p<0.05. Three basic hemodynamic parameters were calculated: rheographic index, period of maximum filling, index of elastic modulus. All patients underwent visual acuity testing and ocular hemodynamics examination by transpalpebral rheoophthalmography before and after LILT. The course of treatment consisted of 10 daily sessions of 10 min each. 6–18 months before LILT, all patients underwent surgical treatment (sinus trabeculectomy) and a course of conservative therapy (3 months after glaucoma surgery). The LILT was performed to16 patients (25 eyes) aged 58–78 years with II (group 1) and III (group 2) stages of primary open-angle glaucoma and normalized level of intraocular pressure without anti-hypertensive regimen. The aim of the investigation was to evaluate the clinical efficacy of low-intensity laser therapy (LILT) using HeNe laser-based MACDEL-08 device in complex treatment of primary open-angle glaucoma. ![]()
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